ALDH3A2 Knockout cell line (THP-1)

ALDH3A2 Knockout cell line (THP-1) Order

Catalog#

YKO-H1166

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

Detailed Product Information

Product category

KO Cell Lines

cell name

ALDH3A2 Knockout cell line (THP-1)

Cell Morphology

Monocyte,suspension

Passage ratio

1：2-1：4

Species

Human

Gene Name

ALDH3A2

Gene ID

224

Gene Information

NCBI

Construction method

Electroporation/Lentivirus

Mycoplasma test

negative

Culture method

90%RPMI-1640+10%FBS

Target region

Knockout region: Exon 1 (based on transcript ALDH3A2-201)
Validation: PCR+Sanger Sequencing

STR Profile

Loci	STR Info (Sample Cell) Sample Cell Line:THP-1			STR Info (Cell bank) Cell Line:THP-1
Loci	Allele1	Allele2	Allele3	Allele1	Allele2	Allele3
D5S818	11	12		11	12
D13S317	13	13		13	13
D7S820	10	10		10	10
D16S539	11	12		11	12
VWA	16	16		16	16
TH01	8	9.3		8	9.3
AMEL	X	Y		X	Y
TPOX	8	11		8	11
CSF1PO	11	13		11	13
D12S391	19	19
FGA	24	25
D2S1338	17	18
D21S11	30	31.2
D18S51	13	14
D8S1179	10	14
D3S1358	15	17
D6S1043	14	14
PENTAE	11	15
D19S433	12.2	13
PENTAD	10	12
D1S1656	16	17

* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the ALDH3A2 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the THP-1 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, ALDH3A2 gene knockout ALDH3A2 Knockout cell line (THP-1) will be expanded and cryopreserved.

Knockout Cell Line Work Flow and Validatio

ALDH3A2 Knockout cell line (THP-1)

Catalog#

YKO-H1166

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

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